How to use: (1) Preheat the instrument. Open the sample compartment cover (the light door closes automatically). Turn on the power, the indicator light is on, and the instrument is warmed up for 20 minutes. The selector switch is placed in the “T†knob so that the number is displayed as “00.0â€. (2) Rotate the wavelength handwheel to align the desired wavelength with the tick mark. (3) Place the cuvette containing the solution in the colorimetric frame and place the reference solution on the light path. (4) Cover the sample chamber cover and adjust the light transmittance “100%T†knob so that the digital display is “100.0Tâ€. (If the display is less than 100%T, press it once. (5) Measurement of absorbance A: After the instrument adjusts T to 0 and 100%, the selector switch is switched to the A zero knob, and the digital display should be “.000â€. Then pull out the lever to place the measured solution into the optical path, and the digital display value is the absorbance A of the sample. (6) After the measurement is completed, open the sample chamber cover and then cut off the power supply. The cuvette should be cleaned. Store and store.
1. Can the spectrophotometer measure the light transmittance?
2. When is the sensitivity adjustment of the spectrophotometer used? What is the difference between him and the method of using different cuvettes?
3. Can the control in the cuvette be used all day long? Using distilled water
4, how much to put in the sample in the cuvette, I remember it seems to be 1 cm from the side of the cuvette
5. Can a spectrophotometer be able to measure the data required for two wavelengths? There is an error in adjusting the wavelength of the light source in this way. Is such data still related to the reference?
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2. Sensitivity adjustment really needs to adjust the sensitivity when used. . . . For example, if the absorbance display is too low, the sensitivity can be adjusted to a different point. The color of the cuvette is different. According to the formula, the width can be converted proportional to the absorbance.
3. Never used it all day. . . Distilled water should be covered
4. Cuvette volume 2/3
5. Note that each measurement must be zeroed so that you can
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